Andriy GnylorybovM.Amosov National Institute of Cardiovascular Surgery of NAMS, Ukraine
Title: The role of regulatory micro-RNAs in inflammatory processes and production of tumor necrosis factor-alpha in patients with rheumatoid arthritis
Micro-RNAs are fundamental agents of post-transcriptional control of gene expression. In recent years many works have appeared on the possible role of micro-RNAs in rheumatoid arthritis (RA). Studies of the role of micro-RNA and the relationship with the synthesis of tumor necrosis factor-?(TNF-?) are very promising for understanding the pathogenesis and treatment of RA and other autoimmune diseases. The purpose of the research was to study the role of regulatory micro-RNAs in inflammatory processes and the possible connection with the production of TNF-? in patients with RA. Materials and methods. 29 patients with active RA and 20 healthy individuals (control) were examined. All subjects were examined for 16 micro-RNAs. The choice of micro-RNA was based on previous studies and theoretical conclusions (according to the miRWalk database). Rheumatoid factor, the level of antibodies to cyclic peptides containing citrulline, C-reactive protein (?RP), levels of TNF-? (se-rum, spontaneous, and stimulated) were determined in the blood of patients. Results. Statistical analysis demonstrated significant overexpression of miR-221, miR-203, miR-146b, miR-132, miR-21 and miR-17-3p and inhibition of miR-223 synthesis in RA patients. The activation of TNF-? synthesis at rest and the increased production of TNF-? by mononuclear cells after stimulation in RA were shown.Differences in the levels of relative expression of some micro-RNAs between seropositive and seronegative groups of RA patients were found, but only hyperexpression of miR-155 was highly reliable. For the first time, a possible relationship between TNF-? production and miR-29 and miR-155 micro-RNAs, as well as a correlation between miR-16, miR-99b and miR-203 and CRP levels, was revealed. Conclusions. The obtained data on the profile of micro-RNAs in RA makes it possible to distinguish the most “interesting” micro-RNAs for further study of pathogenesis, their role in inflammation, to study the choice of TNF-? inhibitors, and predicting the effectiveness of that treatment.
Andriy Gnylorybov has completed his post-graduate DM in rheumatology in National State Medical University, Ukraine. He organized scientific laboratory and a Department of Fundamental Research at the Institute of Emergency and Reconstructive Surgery, Ukraine. Some of his research has been recognized as the best scientific research in National Academy of Sciences, Ukraine. Professor Andriy Gnylorybov worked as a Deputy Director for scientific work at an academic Institute of National Academy of Science, Ukraine. Working in this position, he was engaged in the organization of scientific and practical work. He is currently working on a project on fundamental and clinical research on the pathogenesis of major adverse cardiovascular events in rheumatic disease patients and the role of platelet activation immunophenotype and role of epigenetic factors (micro-RNA) in these complications. Areas of scientific interest: International Clinical Trials in Target Treatment of Rheumatic Diseases (Rheumatoid Arthritis, Spondylarthritis, Gout, Osteoarthritis, SLE); Regenerative Medicine (Treatment of Rheumatic Diseases with Autological Stem Cells, Experimental Treatments of Myocardial Infarction in vivo); Mechanism of Major cardiovascular events in patients with Rheumatic Diseases. He has over 230 publications that have been cited over 150 times. He has been serving as an editorial board member of several reputed journals.